Dulbecco’s MEM

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance

Light orange, homogeneous, free flowing powder.

Solubility

Pink, clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 12.048g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Chloride•2H2O	0.154
Cupric Sulfate•H2O	0.0000013
Ferric Nitrate•9H2O	0.00005
Ferrous Sulfate•7H2O	0.000417
Magnesium Chloride•6H2O	0.0612
Magnesium Sulfate	0.04884
Potassium Chloride	0.3118
Sodium Chloride	6.996
Sodium Phosphate dibasic	0.07102
Sodium Phosphate monobasic	0.0543
Zinc Sulfate•7H2O	0.000432

Amino Acids

L-Alanine	0.00445
L-Arginine•HCl	0.1475
L-Asparagine•H2O	0.0075
L-Aspartic Acid	0.00665
L-Cystine•2HCl	0.03129
L-Cysteine•HCl•H2O	0.01756
L-Glutamic Acid	0.00735
L-Glutamine	0.365
Glycine	0.01875
L-Histidine•HCl•H2O	0.03148
L-Isoleucine	0.05447
L-Leucine	0.05905
L-Lysine•HCl	0.09125
L-Methionine	0.01724
L-Phenylalanine	0.03548
L-Proline	0.01725
L-Serine	0.02625
L-Threonine	0.05345
L-Tryptophan	Absent
L-Tyrosine•2Na•2H20	0.05579
L-Valine	0.05285

Vitamins

Biotin	0.0000035
Choline Chloride	0.00898
Vitamin B12	0.00068
Folic Acid	0.00266
i-inositol	0.0126
Nicotinamide	0.00202
D-Pantothenic Acid•Ca	0.00224
Pyridoxine•HCl	0.000031
Pyridoxal Hydrochloride	0.002
Riboflavin	0.000219
Thiamine•HCl	0.00217

Other

D-Glucose	3.15
Hypoxanthine(Na)	0.0021
Linoleic Acid	0.000042
Lipoic Acid	0.000105
Phenol Red	0.0081
Putrescine•HCl	0.000081
Pyruvic Acid, Sodium	0.055
Thymidine	0.000365

Total:

12.048g/Liter

References

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).

USBio References

1. Tomek P., et al. 2018. Imprinted and ancient gene: a potential mediator of cancer cell survival during tryptophan deprivation. Cell Commun Signal. 16(1):88. doi: 10.1186/s12964-018-0301-7.

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D9807-04 Dulbecco’s MEM (DMEM) F-12 w/o Tryptophan (Powder)

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