Dulbecco’s MEM

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.

Appearance

Beige to light orange, homogeneous, free flowing powder

Solubility

Yellow-orange, clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 15.6g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as

Inorganic Salts

Calcium Chloride•2H20	154.52
Cupric Sulfate•5H2O	0.001245
Ferric Nitrate•9H2O	0.05
Ferrous Sulfate•7H2O	0.417
Magnesium Chloride	28.66
Magnesium Sulfate	48.84
Potassium Chloride	311.8
Sodium Chloride	6996
Sodium Phosphate Dibasic	71.02
Sodium Phosphate Monobasic	54.3
Zinc Sulfate•7H2O	0.4315

Amino Acids

L-Alanine	4.45
L-Arginine	121.97
L-Asparagine•H2O	7.5
L-Aspartic Acid	6.65
L-Cysteine•HCI•H2O	17.65
L-Cystine•2HCI	31.29
L-Glutamic Acid	7.35
L-Glutamine	365.1
Glycine	18.75
L-Histidine HCl•H2O	31.48
L-Isoleucine	54.47
L-Leucine	59.05
L-Lysine•HCI	91.25
L-Methionine	17.24
L-Phenylalanine	35.48
L-Proline	17.25
L-Serine	26.25
L-Threonine	53.45
L-Tryptophan	9.02
L-Tyrosine•2Na•2H20	55.79
L-Valine	52.85

Vitamins

Biotin	0.0035
Choline Chloride	8.98
Vitamin B12	0.68
Folic Acid	2.66
i-Inositol	Absent
Nicotinamide	2.02
D-Pantothenic Acid•Ca	2.24
Pyridoxal HCI	2
Pyridoxine HCI	0.031
Riboflavin	0.219
Thiamine HCI	2.17

Other

Thymidine	0.365
D-Glucose	3150
HEPES Free Acid	3574
Hypoxanthine	2.1
Linoleic Acid	0.042
Lipoic Acid	0.105
Phenol Red	8.63
Putrescine•2HCl	0.081
Sodium Pyruvate	55

Total:

15.6g/liter

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D9811-13 Dulbecco’s MEM (DMEM) Ham’s F-12 w/HEPES w/o Inositol (Powder)

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