Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Components shown as | mg/liter |
Calcium Chloride Dihydrate | Absent |
Cupric Sulfate•5H2O | 0.002 |
Ferrous Sulfate•7H2O | 0.80 |
Magnesium Chloride•6H2O | 106.00 |
Magnesium Sulfate•7H2O | 393.00 |
Potassium Chloride | 285.00 |
Potassium Dihydrogen Phosphate | 59.00 |
Sodium Chloride | 7530.00 |
Sodium Phosphate Dibasic•7H2O | 218.00 |
Zinc Sulfate•7H2O | 0.14 |
L-Alanine | 17.80 |
L-Arginine | 421.30 |
L-Asparagine•H2O | 30.00 |
L-Aspartic Acid | 26.60 |
L-Cysteine•HCI•H2O | 70.04 |
L-Cystine | Absent |
L-Glutamic Acid | 29.40 |
Glycine | 15.00 |
L-Histidine HCl•H2O | 41.90 |
L-Isoleucine | 7.90 |
L-Leucine | 26.20 |
L-Lysine•HCI | 73.10 |
L-Methionine | 8.90 |
L-Phenylalanine | 9.90 |
L-Proline | 69.10 |
L-Serine | 21.00 |
L-Threonine | 23.80 |
L-Tryptophan | 4.10 |
L-Tyrosine | 10.90 |
L-Valine | 23.40 |
Biotin | 0.07 |
Choline Chloride | 13.96 |
Cyanocobalamin (Vitamin B12) | 1.36 |
Folic Acid | 1.32 |
I-inositol | 18.00 |
DL-Thioctic acid | 0.21 |
Nicotinamide | 0.04 |
D-Pantothenic Acid•Na | 0.48 |
Pyridoxine HCI | 0.06 |
Riboflavin | 0.04 |
Thiamine HCI | 0.21 |
Thymidine | 0.70 |
D-Glucose | 4500.00 |
Hypoxanthine | 4.00 |
Methyl Linoleate | Absent |
Phenol Red | Absent |
Putrescine•HCl | 0.30 |
Sodium Pyruvate | 220.00 |
Total: | 14.28g/L |
Product | Size | List | Your Price | Qty | Ext Price | ||||
---|---|---|---|---|---|---|---|---|---|
Subtotal: | Subtotal: | ||||||||
Subtotal: | Subtotal: | ||||||||
Total Coupon Savings: | Total Coupon Savings: | () | |||||||
Your cart is currently empty. | |||||||||
- Coupon Code |