Technical Data

D9811-17
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Dulbecco’s MEM (DMEM) Ham’s F-12 3:1, Calcium Free w/Sodium Pantothenate (Powder)

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.
Appearance
Light orange to orange, homogeneous, free flowing powder
Solubility
Orange to salmon, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Dissolve 12.53g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.8 to 3.7 g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/liter
Inorganic Salts:mg/L
Cupric Sulfate•5H2O0.000625
Ferric Nitrate•9H2O0.075
Ferrous Sulfate•7H2O0.2085
Magnesium Chloride14.39
Magnesium Sulfate73.2525
Potassium Chloride356
Sodium Chloride6699.75
Sodium Phosphate Dibasic35.51
Sodium Phosphate Monobasic•H2O 93.75
Zinc Sulfate•7H2O0.21575
Amino Acids
L-Alanine2.25
L-Arginine•HCl115.75
L-Asparagine•H2O3.7525
L-Aspartic Acid3.325
L-Cysteine•HCl•H2O8.75
L-Cystine•2HCI47.25
L-Glutamic Acid3.675
L-Glutamine474.5
Glycine24.3775
L-Histidine•HCl•H2O36.74
L-Isoleucine79.735
L-Leucine82.025
L-Lysine•HCl118.625
L-Methionine23.62
L-Phenylalanine50.74
L-Proline8.625
L-Serine34.125
L-Threonine74.225
L-Tryptophan12.51
L-Tyrosine•Na2•2H2O79.945
L-Valine73.425
Vitamins
D-Biotin0.001825
Choline Chloride6.49
Vitamin B-12 0.34
Folic Acid3.33
myo-Inositol9.9
Niacinamide3.00925
D-Sodium Pantothenate3.12
Pyridoxine•HCl3.0155
Riboflavin0.3095
Thiamine•HCl3.085
Other
Thymidine0.1825
D-Glucose3825.5
Hypoxanthine1.02
Linoleic Acid0.021
Thioctic Acid (Lipoic acid)0.0525
Phenol Red11.575
Putrescine•2HCl0.04025
Sodium Pyruvate27.5
Total:12.53g/L

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974). 6. Kaighn, M.E., (1973) in “Tissue Culture Methods and Applications,” Kruse, Jr., 7. P.F. and Patterson, Jr., M.D., eds., Academy Press, NY, 54-58. 8. Robey, P.G. and Termine, J.D. (1985) Calcif. Tissue Int. 37:453-460.|
USBio References
No references available
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