Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
| Components shown as mg/Liter |
| Calcium Chloride•2H2O | 265 |
| Ferric Nitrate•9H2O | 0.10 |
| Magnesium Sulfate | 97.67 |
| Potassium Chloride | 400.00 |
| Sodium Chloride | 6400.00 |
| Sodium Phosphate Monobasic, Anhy | 109.00 |
| L-Arginine•HCl | Absent |
| L-Cystine•2HCl | 62.60 |
| Glycine | 30.00 |
| L-Glutamine | Absent |
| L-Histidine•HCl•H2O | 42.00 |
| L-Isoleucine | 105.00 |
| L-Leucine | 105.00 |
| L-Lysine•HCl | 146.00 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Serine | 42.00 |
| L-Threonine | 95.00 |
| L-Tryptophan | 16.00 |
| L-Tyrosine•2Na•H2O | 103.79 |
| L-Valine | 94.00 |
| Choline Chloride | 4.00 |
| FolicAcid | 4.00 |
| myo-Inositol | 7.20 |
| Niacinamide | 4.00 |
| D-Pantothenic Acid•Ca | 4.00 |
| Pyridoxal•HCl | 4.00 |
| Riboflavin | 0.40 |
| Thiamine•HCl | 4.00 |
| D+Glucose | 4500.00 |
| Pyruvic Acid, Sodium | Absent |
| Phenol Red | Absent |
| Total: | 12.74g/Liter |
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