Technical Data

D9815-01A
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Dulbecco’s MEM (DMEM) Base w/o L-Glutamine, Phenol Red (Powder)

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
Off-white to tan, homogeneous, free flowing powder
Solubility
Colorless to light yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Dissolve 9.43g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Chloride•2H2O0.265
Ferric Nitrate•9H2O0.0001
Magnesium Sulfate0.09767
Potassium Chloride0.40
Sodium Chloride6.40
Sodium Phosphate Mono 0.109
Amino Acids
L-Arginine•HCl0.084
L-Cystine0.0626
L-Glutamine Absent
Glycine0.03
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.03
L-Phenylalanine0.066
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins
Choline Chloride0.004
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other
Glucose 1.000
Phenol Red Absent
Sodium Pyruvate 0.110
Total: 9.43g/liter

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).
USBio References
No references available
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