Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. EMM2 is used for vegetative growth. This formulation does not contain Ammonium Chloride, a Nitrogen source.
| Components shown as g/Liter |
| Potassium Hydrogen Phthalate | 3.00 |
| Sodium Phosphate Dibasic | 2.20 |
| Ammonium Chloride | Absent |
| Glucose | 20.00 |
| Magnesium Chloride•6H2O | 1.05 |
| Calcium Chloride•2H2O | 0.0147 |
| Potassium Chloride | 1.00 |
| Sodium Sulfate | 0.04 |
| Pantothenic Acid | 0.001 |
| Nicotinic Acid | 0.01 |
| Inositol | 0.01 |
| Biotin | 0.00001 |
| Boric Acid | 0.0005 |
| Manganese Sulfate | 0.0004 |
| Zinc Sulfate•7H2O | 0.0004 |
| Ferrous Chloride | 0.0002 |
| Molybdic Acid | 0.00004 |
| Potassium Iodide | 0.0001 |
| Copper Sulfate •5H2O | 0.00004 |
| Citric Acid | 0.001 |
| Total: | 27.33g/Liter |
| Edinburgh Minimal Medium (EMM 2) | |
| 3g/L Potassium hydrogen phthalate (14.7mM) | |
| 2.2g/L Sodium phosphate dibasic (15.5mM) | |
| 5g/L Ammonium chloride (93.5mM) | |
| 2% (w/v) Glucose(111mM) | |
| 20ml/L Salts (50X stock) | |
| 1ml/L Vitamins (1000X stock) | |
| 0.1ml/L Minerals (10,000X stock) |
| 52.5g/L Magnesium chloride•6H2O (0.26M) | |
| 0.735g/L Calcium chloride•2H2O (4.99mM) | |
| 50g/L Potassium chloride (0.67M) | |
| 2g/L Sodium sulfate (14.1mM) |
| 1g/L Pantothenic acid (4.20mM) | |
| 10g/L Nicotinic acid (81.2mM) | |
| 10g/L Inositol (55.5mM) | |
| 10mg/L Biotin (40.8uM) |
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