-Added to Storage and Stability
Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomy4/1/25: Changed pH: 6.5
5/12/05-changed Storage: RT -DF
5/12/5-Changed pH: 5.5 ± 0.4 per DF. -JRL
04/27/05-Removed Catalog Master Particle Distribution (microns, um): As reported -VV
06/28/04-Remvoed from Desc 1: All lots are tested for thiamine content using a beta-galactosidase/lacZ system. -VV
10.28.99 Changed List/Siteces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.
Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.
Appearance
White to light tan, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
Directions per Liter
Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/Liter |
Base
Ammonium Chloride | 5.000 |
Glucose | 20.000 |
Sodium Acetate•3H2O | 2.200 |
Salt Stock
Calcium Chloride•2H2O | 0.0147 |
Magnesium Chloride•6H2O | 1.050 |
Potassium Chloride | 1.000 |
Sodium Sulfate | 0.040 |
Vitamin Stock
Biotin | 0.00001 |
Inositol | 0.010 |
Nicotinic Acid | 0.010 |
Pantothenic Acid•Ca | 0.001 |
Mineral Stock
Boric Acid | 0.0005 |
Citric Acid | 0.001 |
Copper Sulfate•5H2O 0.00004 |
Ferrous Chloride 0.0002 |
Manganese Sulfate | 0.0004 |
Potassium Iodide | 0.0001 |
Molybdic Acid 0.00004 |
Zinc Sulfate•7H2O | 0.0004 |
Note: The above formulation was derived from the following referenced formula
Edinburgh Minimal Medium (EMM 2) |
3g/L | Potassium hydrogen phthalate (14.7mM) |
2.2g/L | Sodium phosphate dibasic (15.5mM) |
5g/L | Ammonium chloride (93.5mM) |
2% (w/v) | Glucose (111mM) |
20ml/L | Salts (50X stock) |
1ml/L | Vitamins (1000X stock) |
0.1ml/L | Minerals (10,000X stock) |
50X Salts
52.5g/L | Magnesium chloride•6H2O (0.26M) |
0.735g/L | Calcium chloride•2H2O (4.99mM) |
50g/L | Potassium chloride (0.67M) |
2g/L | Sodium sulfate (14.1mM) |
1000X Vitamins
1g/L | Pantothenic acid (4.20mM) |
10g/L | Nicotinic acid (81.2mM) |
10g/L | Inositol (55.5mM) |
10mg/L | Biotin (40.8uM) |