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E2205-20 Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)

Specifications
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

-Added to Storage and Stability
Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomy4/1/25: Changed pH: 6.5
5/12/05-changed Storage: RT -DF
5/12/5-Changed pH: 5.5 ± 0.4 per DF. -JRL
04/27/05-Removed Catalog Master Particle Distribution (microns, um): As reported -VV
06/28/04-Remvoed from Desc 1: All lots are tested for thiamine content using a beta-galactosidase/lacZ system. -VV
10.28.99 Changed List/Siteces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.
Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.
Appearance
White to light tan, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Directions per Liter
Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/Liter
Base
Ammonium Chloride5.000
Glucose20.000
Sodium Acetate•3H2O2.200
Salt Stock
Calcium Chloride•2H2O0.0147
Magnesium Chloride•6H2O1.050
Potassium Chloride1.000
Sodium Sulfate0.040
Vitamin Stock
Biotin 0.00001
Inositol0.010
Nicotinic Acid0.010
Pantothenic Acid•Ca0.001
Mineral Stock
Boric Acid0.0005
Citric Acid0.001
Copper Sulfate•5H2O 0.00004
Ferrous Chloride 0.0002
Manganese Sulfate0.0004
Potassium Iodide0.0001
Molybdic Acid 0.00004
Zinc Sulfate•7H2O0.0004
Total: 29.328g/L
Note: The above formulation was derived from the following referenced formula
Edinburgh Minimal Medium (EMM 2)
3g/LPotassium hydrogen phthalate (14.7mM)
2.2g/LSodium phosphate dibasic (15.5mM)
5g/LAmmonium chloride (93.5mM)
2% (w/v)Glucose (111mM)
20ml/LSalts (50X stock)
1ml/LVitamins (1000X stock)
0.1ml/LMinerals (10,000X stock)
50X Salts
52.5g/LMagnesium chloride•6H2O (0.26M)
0.735g/LCalcium chloride•2H2O (4.99mM)
50g/LPotassium chloride (0.67M)
2g/LSodium sulfate (14.1mM)
1000X Vitamins
1g/LPantothenic acid (4.20mM)
10g/LNicotinic acid (81.2mM)
10g/LInositol (55.5mM)
10mg/LBiotin (40.8uM)
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