Endothelial Basal Medium MCDB 131 (Powder)

E3000-01B

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Endothelial Basal Medium MCDB 131 (Powder)

Base powder without supplements

MCDB 131 was developed by Knedler and Ham as a reduced serum-supplemented medium for the culture of human microvascular endothelial cells. This formulation has been reported to support clonal growth of human omental microvascular cells when supplemented with 0.7% dialyzed FBS, 10ng/mL EGF, and 1ug/mL hydrocortisone.

Appearance

Pale orange to orange, homogeneous, free-flowing powder

Solubility

Pink to red or orange, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 11.53g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Media Formulation

Components shown as

mg/L

Inorganic Salts

Ammonium Metavanadate	0.0006
Ammonium Molybdate•4H2O	0.0037
Calcium Chloride•2H2O	235
Cupric Sulfate•5H2O	0.0012
Ferrous Sulfate•7H2O	0.283
Magnesium Sulfate•7H2O	2464
Manganese Sulfate•H20	0.0002
Nickel Chloride•6H2O	0.000071
Potassium Chloride	298
Sodium Chloride	6430
Sodium Meta Silicate•9H2O	2.8
Sodium Phosphate Dibasic•7H2O	134
Zinc Sulfate•H2O	0.0003

Amino Acids

L-Alanine	2.7
L-Arginine Hydrochloride	63.2
L-Asparagine•H2O	15
L-Aspartic Acid	13.3
L-Cysteine•2HCl.•H2O	35
L-Glutamic Acid	4.4
Glycine	2.3
L-Histidine Hydrochloride•H2O	42
L-Isoleucine	66
L-Leucine	131
L-Lysine Hydrochloride	182
L-Methionine	15
L-Phenylalanine	33
L-Proline	11.5
L-Serine	32
L-Threonine	12
L-Tryptophan	4.1
L-Tyrosine	18.1
L-Valine	117

Vitamins

Biotin	0.0073
Choline Chloride	14
Vitamin B12	0.0136
Folinic Acid Calcium	0.6
i-Inositol	7.2
Niacinamide	6.1
D-Calcium Pantothenate	12
Pyridoxine Hydrochloride	2.1
Riboflavin	0.0038
Thiamine Hydrochloride	3.4

Other Components

Adenine	0.135
Thymidine	0.024
D-Glucose	1000
Lipoic Acid	0.0021
Phenol Red	12.4
Putrescine•2HCl	0.0002
Selenious Acid	0.0038
Sodium Pyruvate	110

Total:

11.53g/L

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References

1. Lee DK and Nevo O. Microvascular endothelial cells from preeclamptic women exhibit altered expression of angiogenic and vasopressor factors. 2016. Am J Physiol Heart Circ Physiol. 310(11):H1834-H1841. doi: 10.1152/ajpheart.00083.2016.|2. Di Meglio, F. et al. Localization and origin of cardiac CD117-positive cells: identification of a population of epicardially-derived cells in adult human heart. 2010. Italian J. of Anatomy and Embryology 115: 71-78. |3. Prisco AR, et al. TNFα Increases Endothelial Progenitor Cell Adhesion to the Endothelium by Increasing Bond Expression and Affinity. Am J Physiol Heart Circ Physiol. 2014 Dec 24:ajpheart.00496.2014. doi: 10.1152/ajpheart.00496.2014.

Technical Data

E3000-01B

Grade

EU Commodity Code

Shipping Temp

Storage Temp

Endothelial Basal Medium MCDB 131 (Powder)

Base powder without supplements

References

USBio References