I1904-89B1-ML490
Grade
PurifiedPurity
PurifiedForm
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.Conjugate
MaxLight™490Specificity
Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzeeEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
IgG4 (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen and paraffin sections. Optimal dilutions to be determined by the researcher.
Hybridoma
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Jefferis, R. et al. (1985). Immunology Letters. 10:223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34:271-8. 3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders. J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology. 72:94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9:29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46:195-8.USBio References
No references available