K1778-APC
Grade
PurifiedPurity
Purified by ammonium sulfate precipitation.Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).Conjugate
APCSpecificity
Recognizes human Kindlin-2. Species sequence homology: mouse, rat, bovine.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
BSA Free
Kindlin-2 (Fermitin family homolog 2, KIND2, Kindlin-2, MIG2, mig-2, MIG-2, Mitogen-inducible gene 2 protein, PH domain-containing family C member 1, Pleckstrin homology domain-containing family C member 1, PLEKHC1, UNC112, UNC112B, FERMT2) (APC)
The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
