K1778-FITC
Grade
PurifiedPurity
Purified by ammonium sulfate precipitation.Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).Conjugate
FITCSpecificity
Recognizes human Kindlin-2. Species sequence homology: mouse, rat, bovine.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Kindlin-2 (Fermitin family homolog 2, KIND2, Kindlin-2, MIG2, mig-2, MIG-2, Mitogen-inducible gene 2 protein, PH domain-containing family C member 1, Pleckstrin homology domain-containing family C member 1, PLEKHC1, UNC112, UNC112B, FERMT2) (FITC)
The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
