L-15ex medium is a minimal medium based on Leibovitz L-15 basal cell culture medium. This medium, which was developed by Schirmer, et al. for studying the photocytotoxicity of polycyclic aromatic hydrodcarbons (PAHs) to the rainbow trout gill cell line, RTgill-W1 contains only salts, pyruvate and galactose. The simplicity of the medium favors expression of cellular responses to toxicants because protective molecules such as anti-oxidants are absent.
Components shown as g/L |
Calcium Chloride | 0.08 |
Galactose | 0.51 |
Magnesium Chloride | 0.11 |
Magnesium Sulfate | 0.11 |
Phenol Red | Absent |
Potasssium Chloride | 0.23 |
Potassium Phosphate, Monobasic | 0.03 |
Sodium Chloride | 4.53 |
Sodium Phosphate, Dibasic | 0.11 |
Sodium Pyruvate | 0.31 |
Total Weight: | 6.02g/L |
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