LB Broth Lennox (Powder)
Lennox L broth base
LB Lennox, a nutritionally rich medium, was developed by Lennox for the growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950’s (1,2,3). These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5).
The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin™.
Principles of the Procedure
Peptides and peptones are provided by Tryptone. Vitamins (including B vitamins) and certain trace elements are provided by Yeast Extract. Sodium ions for transport and osmotic balance are provided by sodium chloride.
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.
Homogeneous: Proprietary blending technology ensures complete mixing of components.
Appearance
Light tan, homogeneous, free flowing powder
Solubility
Pale yellow to yellow, clear, complete
Companion Products
Terrific Broth is a richer media that would be suitable if higher cell densities and higher yields for plasmids or protein purifications are necessary. T2810: Terrific Broth (Powder) T2810-05: Terrific Broth, Complete with Carbon Source (Powder) T2810-10: Terrific Broth, Modified for Genomics (Powder) T2810-11: Terrific Broth, Modified for Fermentation, non-animal (Powder)
L1500: LB Agar Lennox (Powder) L1505: LB Broth Lennox (Powder) L1510: LB Top Agar Lennox (Powder) P9102-40H: Protein Expression Media, LB Broth Lennox (Powder) pEX™ P9102-40H1: Protein Expression Media, LB Broth Lennox (Liquid) pEX™
L1515: LB Agar Miller (Powder) L1520: LB Broth Miller (Powder) L1525: LB Top Agar Miller (Powder) P9102-40G: Protein Expression Media, LB Broth Miller (Powder) pEX™ P9102-40G1: Protein Expression Media, LB Broth Miller (Liquid) pEX™
L1530: L-Broth (Luria Broth) (Powder)
Directions per Liter
Dissolve 20g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Sodium Chloride | 5 |
| Tryptone | 10 |
| Yeast Extract | 5 |
| Total: | 20g/liter |
