Technical Data

M1010
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
M9 Minimal Salts (Powder)
Sodium Phosphate dibasic, Potassium Phosphate mono, Ammonium Chloride, Sodium Chloride

Buffered salt formulation used as a base to prepare minimal media for propagation of E. coli.

Appearance
White, homogeneous and free flowing powder
Solubility
Colorless, clear, complete
pH
6.8-7.4
Directions per Liter
Dissolve 10.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as g/liter
Ammonium Chloride1.0
Potassium Phosphate mono3.0
Sodium Chloride0.5
Sodium Phosphate dibasic6.0
Total10.5g/liter
Note
Calcium chloride is not included since it is optional in the preparation of M9. If desired, add 15mg/liter.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Woolston BM, et al. Development of a formaldehyde biosensor with application to synthetic methylotrophy. Biotechnol Bioeng. 2018. 115(1):206-215. doi: 10.1002/bit.26455.
USBio References
1. Woolston BM, et al. Development of a formaldehyde biosensor with application to synthetic methylotrophy. Biotechnol Bioeng. 2018. 115(1):206-215. doi: 10.1002/bit.26455.
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net