Marine Agar (Zobell)
Suitable for use in culturing heterotropic marine bacteria. This formulation simulates the mineral composition of sea water.
Appearance
Off-white to tan, homogeneous, free flowing powder
Solubility
Light yellow, opalescent gel forms in Petri plates after autoclaving.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 52.35 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Formulation per Liter | g/Liter |
| Bacti Agar | 15.00 |
| Ammonium Nitrate | 0.0016 |
| Boric Acid | 0.022 |
| Calcium Chloride | 1.80 |
| Ferric Citrate | 0.10 |
| Magnesium Chloride, anhydrous | 5.90 |
| Peptone | 5.00 |
| Potassium Bromide | 0.08 |
| Potassium Chloride | 0.55 |
| Sodium Bicarbonate | 0.16 |
| Sodium Chloride | 19.45 |
| Sodium Fluoride | 0.0024 |
| Sodium Phosphate Dibasic | 0.008 |
| Sodium Sulfate | 3.24 |
| Sodium Silicate | 0.004 |
| Strontium Chloride | 0.034 |
| Yeast Extract | 1.00 |
