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M3398-55M-ML550 5-Methylcytidine (MaxLight 550)

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
Purity
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Conjugate
MaxLight™550
Specificity
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Specific to the methylated base and has|minimal reactivity to non-methylated cytidine or cytosine. Species Crossreactivity: human and mouse.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Dot Blot, Immunofluorescence, Flow Cytometry, Western Blot, Methylated DNA Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262. 2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53. 3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693. 4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol. Reprod. Dev. 74: 1514-1524.
USBio References
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