M3853
Grade
Cell Culture GradeEU Commodity Code
38210000Shipping Temp
RTStorage Temp
RT/4°CMEM Alpha Modified w/ L-Glutamine, Ribo, Deoxyribonucleosides, HEPES (Powder)
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in mono-layers.
Appearance
Light orange, homogenous, free flowing powder
Solubility
Yellow, clear, complete
Directions per Liter
Dissolve 16.17g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as mg/liter |
Inorganic Salts
| Calcium Chloride | 264.92 |
| Magnesium Sulfate | 97.67 |
| Potassium Chloride | 400 |
| Sodium Chloride | 6800 |
| Sodium Phosphate Monobasic | 121.74 |
Amino Acids
| L-Alanine | 25 |
| L-Arginine•HCl | 126.98 |
| L-Asparagine | 50 |
| L-Aspartic Acid | 30 |
| L-Cysteine•HCI | 100 |
| L-Cystine•2HCI | 31.29 |
| L-Glutamic Acid | 75 |
| L-Glutamine | 292 |
| Glycine | 50 |
| L-Histidine•HCl | 42 |
| L-Isoleucine | 52.4 |
| L-Leucine | 52.4 |
| L-Lysine•HCI | 72.46 |
| L-Methionine | 15 |
| L-Phenylalanine | 32 |
| L-Proline | 40 |
| L-Serine | 25 |
| L-Threonine | 48 |
| L-Tryptophan | 10 |
| L-Tyrosine•2Na | 51.9 |
| L-Valine | 46 |
Vitamins
| Ascorbic Acid | 50 |
| Biotin | 0.1 |
| Choline Chloride | 1 |
| Vitamin B12 | 1.36 |
| Folic Acid | 1 |
| i-Inositol | 2 |
| Nicotinamide | 1 |
| D-Pantothenic Acid•Ca | 1 |
| Pyridoxal•HCI | 1 |
| Riboflavin | 0.1 |
| Thiamine•HCI | 1 |
Other
| Adenosine | 10 |
| Cytidine | 10 |
| 2’Deoxyadenosine | 10 |
| 2’Deoxycytidine•HCl | 11 |
| 2’Deoxyguanosine | 10 |
| Thymidine | 10 |
| D-Glucose | 1000 |
| Guanosine | 10 |
| HEPES Free Acid | 5958 |
| Lipoic Acid | 0.2 |
| Phenol Red | 10.2 |
| Sodium Pyruvate | 110 |
| Uridine | 10 |
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956) 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955) 5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971) 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)USBio References
No references available