M3861-20
Grade
Cell Culture GradeEU Commodity Code
38210000Shipping Temp
RTStorage Temp
RT/4°CMEM Eagle D-Valine Modification w/L-Glutamine, D-Valine (Powder) (EMEM)
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.
Appearance
Orange to red, homogeneous, free flowing powder
Solubility
Red orange-yellow, clear, complete
Directions per Liter
Dissolve 9.62g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
Inorganic Salts
| Calcium Chloride•2H2O | 0.265 |
| Magnesium Sulfate | 0.09767 |
| Potassium Chloride | 0.40 |
| Sodium Chloride | 6.80 |
| Sodium Phosphate Monobasic | 0.122 |
Amino Acids
| L-Arginine•HCl | 0.126 |
| L-Cystine•2HCl | 0.0313 |
| L-Glutamine | 0.292 |
| L-Histidine•HCl•H2O | 0.042 |
| L-Isoleucine | 0.052 |
| L-Leucine | 0.052 |
| L-Lysine•HCl | 0.0725 |
| L-Methionine | 0.015 |
| L-Phenylalanine | 0.032 |
| L-Threonine | 0.048 |
| L-Tryptophan | 0.010 |
| L-Tyrosine•2Na•2H2O | 0.0519 |
| D-Valine | 0.092 |
Vitamins
| Choline Chloride | 0.001 |
| Folic Acid | 0.001 |
| myo-Inositol | 0.002 |
| Niacinamide | 0.001 |
| D-Pantothenic Acid•Ca | 0.001 |
| Pyridoxal•HCl | 0.001 |
| Riboflavin | 0.0001 |
| Thiamine•HCl | 0.001 |
Other
| D-Glucose | 1.00 |
| Phenol Red, Sodium | 0.011 |
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Pellitteri-Hahn, M. C. et al., (2006) J Proteome Res. 5:2861. 2. Freed, J.K. and Greene, A.S. (2010) Microcirculation 17:259–270. 3. Tribulatti, M.V. et al. (20070 Glycobiology 17:1404-1412. 4. Palumbo, M.O. et al., (2006) Diabetes 55:2595-2601. 5. Chaki, S. Ph.D. Dissertation (2010) etd.ohiolink.edu. 1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955)USBio References
US Biological application references: 1. Pellitteri-Hahn, M. C. et al., (2006) J Proteome Res. 5:2861. 2. Freed, J.K. and Greene, A.S. (2010) Microcirculation 17:259–270. 3. Tribulatti, M.V. et al. (20070 Glycobiology 17:1404-1412. 4. Palumbo, M.O. et al., (2006) Diabetes 55:2595-2601. 5. Chaki, S. Ph.D. Dissertation (2010) etd.ohiolink.edu.