Technical Data

M3873-L
Grade
Cell Culture Grade
Form
Red, clear, complete liquid.
Shipping Temp
Blue Ice
Storage Temp
4°C
MEM w/Earle’s Temin’s Modification|w/L-Glutamine, NaHCO3, antibiotics (Liquid)
pH
As Reported
Endotoxin
≤1EU/ml
Storage and Stability
Store at 4°C. Stable for 6 months after receipt.
Media Formulation
Components shown as mg/liter
Inorganic Salts
Calcium Chloride199.59
Ferric Nitrate0.1
Magnesium Sulfate100
Potassium Chloride400
Sodium Chloride6080
Sodium Phosphate Monobasic121.4
Amino Acids
L-Arginine•HCl253.96
L-Cystine•2Na51.01
L-Glutamine584
Glycine30
L-Histidine•HCl83.76
L-Isoleucine105
L-Leucine105
L-Lysine•HCI145
L-Methionine30
L-Phenylalanine64
L-Serine42
L-Threonine94
L-Tryptophan20
L-Tyrosine•2Na103.79
L-Valine96
Vitamins
Choline Chloride2
Folic Acid2
i-Inositol4
Nicotinamide2
D-Pantothenic Acid•Ca2
Pyridoxal•HCI2
Riboflavin0.2
Thiamine•HCl2
Antibiotics
Penicillin G•Na (~1600u/mg)60.1
Streptomycin Sulfate (~750mcg/mg)104.2
Other
D-Glucose4500
Phenol Red, Sodium10
Sodium Bicarbonate4200

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956). 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976). 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955)|5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971). 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)
USBio References
No references available
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