NCTC 135 Media, Modified
NCTC Media, developed by the Tissue Culture Section, Laboratory of Biology, National Cancer Institute (NCI), Bethesda, MD to establish and maintain the L929 strain of mouse cells.
Appearance
White to light pink, homogenous, free flowing powder
Solubility
Colorless to slight yellow, clear, complete
Directions per Liter
Dissolve 7.89g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 2.2g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Minerals & Coenzymes | mg/Liter |
| Calcium Chloride, anhydrous | 200.00 |
| Coenzyme A•Na | 2.50 |
| Cocarboxylase | 1.00 |
| Ferric Nitrate•9H2O | 0.10 |
| Magnesium Sulfate | 100.00 |
| beta-NAD | 7.00 |
| beta-NADP•Na (NADP) | 1.00 |
| Potassium Chloride | 400.00 |
| Flavin Adenine Dinucleotide•2Na (FAD) | 1.00 |
| Sodium Chloride | 6600.00 |
| Sodium Phosphate, Mono, anhydrous | 50.00 |
| Uridine-5’-Triphosphate (UTP) | 1.00 |
| Vitamins |
| p-Aminobenzoic acid | 0.125 |
| Ascorbic acid (Vitamin C) | 50.00 |
| Biotin (Vitamin H) | 0.025 |
| Choline chloride (Vitamin B7 ) | 1.25 |
| Cyanocobalamin | 0.25 |
| Calciferol (Vitamin D2) | 0.25 |
| Folic acid (Vitamin B9) | 0.025 |
| myo-Inositol | 0.125 |
| Menadione (Vitamin K) | 0.025 |
| Niacinamide (Vitamin B3) | 0.0625 |
| D-Pantothenic Acid, Calcium Salt | 3.025 |
| Pyridoxal HCl | 0.0625 |
| Riboflavin (Vitamin B2) | 0.025 |
| Thiamine (Vitamin B1) | 0.25 |
| Tocopherol (Vitamin E) | 0.025 |
| Retinol (Vitamin A) | 0.25 |
| Amino Acids |
| L-Amino-n-butyric acid | 6.00 |
| L-Alanine | 32.00 |
| L-Arginine | 31.00 |
| L-Asparagine | 8.00 |
| L-Aspartic acid | 10.00 |
| L-Cystine | 10.00 |
| L-Glutamic acid | 8.00 |
| L-Glutamine | 136.00 |
| Glycine | 14.00 |
| L-Histidine | 20.00 |
| L-Hydroxyproline | 4.00 |
| L-Isoleucine | 18.00 |
| L-Leucine | 20.00 |
| L-Lysine | 31.00 |
| L-Methionine | 4.00 |
| L-Ornithine | 7.00 |
| L-Phenylalanine | 17.00 |
| L-Proline | 6.00 |
| L-Serine | 11.00 |
| Taurine | 4.00 |
| L-Threonine | 19.00 |
| L-Trytophan | 18.00 |
| L-Tyrosine | 16.00 |
| L-Valine | 25.00 |
