Technical Data

O8175
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
4°C
Oxgall (Bovine bile, Bacteriological ox bile)

Oxgall is a purified, dried form of ox bile used in bacteriological media formulations for gastro-intestinal tract organisms, eg, enterocossel agar. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium.

Oxbile is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.
Synonyms
Bovine bile, Bacteriological ox bile
CAS No
8008-63-7
Source
Bovine bile
Appearance
Straw to beige or tan, free-flowing homogeneous powder
Solubility (2%)
Gold to amber, clear, complete
pH (2%)
7.0-8.5
Loss on Drying
≤6%
Total Bile Acids
≥50%
Storage and Stability
Store powder at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Stable for 6 months after receipt. Protect from moisture and light by keeping container tightly closed.
Certificate of Origin
USA
Source
Bovine bile
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Vargas LA, et al. Whey protein isolate improves acid and bile tolerances of Streptococcus thermophilus ST-M5 and Lactobacillus delbrueckii ssp. bulgaricus LB-12. J Dairy Sci. 2015 Jan 23. pii: S0022-0302(15)00034-X. doi: 10.3168/jds.2014-88691. |2. The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota. Lagkouvardos I, et al. Nat Microbiol 1, 16131, (2016)|3. Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome.|Brian V Jones et. al., PNAS, 105(36), 13580-13585 (2008)|4. Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model, Philipp Ritter et. al., BMC Microbiology, 9, 87 (2009)
USBio References
1. Vargas LA, et al. Whey protein isolate improves acid and bile tolerances of Streptococcus thermophilus ST-M5 and Lactobacillus delbrueckii ssp. bulgaricus LB-12. J Dairy Sci. 2015 Jan 23. pii: S0022-0302(15)00034-X. doi: 10.3168/jds.2014-8869. |2. Mena B. and Aryana K. Short communication: Lactose enhances bile tolerance of yogurt culture bacteria. 2018. J Diary Sci. 101(3):1957-1959. doi: 10.3168/jds.2017-13919.
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net