PDA; Potato Infusion, Dextrose, Agar
A standard methods medium used to culture yeasts and molds from dairy and other food products.
Appearance
Light beige, homogeneous, free flowing powder
Solubility
Pale to light yellow, clear to slightly hazy and complete with heat
Growth Performance
Note: Growth results obtained from type cultures after incubation at 25-30°C and observed after 2-7 days.
Aspergillus brasiliensis ATCC 16404 Candida albicans ATCC 10231 Saccharomyces cerevisiae ATCC 97 Trichophyton mentagrophytes ATCC 9533
Directions per Liter
Dissolve 39 grams per liter of dH2O, heat to boiling, stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15psi). Cool to 45°C. Acidify medium to pH 3.5 with sterile tartaric acid.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Potato Infusion | 4.0 |
| Dextrose | 20.0 |
| Agar | 15.0 |
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