Pseudomonas Isolation Broth (Powder)
Pseudomonas Isolation Broth is a modification of a formula described by King et al. Pseudomonas Isolation Broth was found useful in the isolation of Pseudomonas from pharmaceutical preparations (lotions and creams). The incorporation of lrgasan (R) inhibits most bacteria but allows the growth of Pseudomonas. The formulation also enhances pyocyanin production.
Appearance
Off-white to tan, homogeneous, free flowing powder
Solubility
Light yellow to tan, clear, complete with heat
Directions per Liter
Dissolve 31.4 grams in 800-900ml of ddH2O containing 20ml of glycerol, stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Irgasan (R) | 0.025 |
| Magnesium Chloride | 1.4 |
| Pancreatic Digest of Gelatin | 20.0 |
| Potassium Sulfate | 10.0 |
