R8999-17
Grade
Cell Culture GradeEU Commodity Code
38210000Shipping Temp
RTStorage Temp
RT/4°CRPMI 1640 Medium ATCC Modification (Powder)
RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
Appearance
Light pink to orange, homogenous, free flowing powder
Solubility
Pink to magenta, clear, complete
Directions per Liter
Dissolve 16.9g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Inorganic Salts
| Calcium Nitrate•4H2O | 100 |
| Magnesium Sulfate | 48.84 |
| Potassium Chloride | 400 |
| Sodium Bicarbonate | 1500 |
| Sodium Chloride | 6000 |
| Sodium Phosphate Dibasic | 800 |
Amino Acids
| L-Arginine | 200 |
| L-Asparagine | 50 |
| L-Aspartic Acid | 20 |
| L-Cystine•2HCl | 65 |
| L-Glutamic Acid | 20 |
| L-Glutamine | 300 |
| Glycine | 10 |
| L-Histidine | 15 |
| L-Hydroxyproline | 20 |
| L-Isoleucine | 50 |
| L-Leucine | 50 |
| L-Lysine•HCl | 40 |
| L-Methionine | 15 |
| L-Phenylalanine | 15 |
| L-Proline | 20 |
| L-Serine | 30 |
| L-Threonine | 20 |
| L-Tryptophan | 5 |
| L-Tyrosine•2Na•2H2O | 29 |
| L-Valine | 20 |
Vitamins
| p-Aminobenzoic Acid | 1 |
| Biotin | 0.2 |
| Choline Chloride | 3 |
| Vitamin B12 | 0.005 |
| Folic Acid | 1 |
| i-Inositol | 35 |
| Niacinamide | 1 |
| D-Pantothenate•Ca | 0.25 |
| Pyridoxine•HCl | 1 |
| Riboflavin | 0.2 |
| Thiamine•HCl | 1 |
Other
| D-Glucose | 4500 |
| Glutathione (reduced) | 1 |
| HEPES | 2383 |
| Phenol Red | 5 |
| Sodium Pyruvate | 110 |
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508. (1976). 3. Moore, G.E. Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research 41-63. (1967, February). 4. Moore, G.E. Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).USBio References
No references available