RPMI 1640 Medium Modified w/o L-Glutamine, Glucose, Thiamine•HCl

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute.

Appearance

Pale pink to orange, homogeneous, free flowing powder

Solubility

Pink to red, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 8.14g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as mg/liter

Inorganic Salts

Calcium Nitrate•4H2O	100
Magnesium Sulfate Anhy	48.8
Potassium Chloride	400
Sodium Chloride	6000
Sodium Phosphate Dibasic	800.7

Amino Acids

L-Arginine HCl	241
L-Asparagine•H2O	56.82
L-Aspartic Acid	20
L-Cystine•2HCl	65.2
L-Glutamine	Absent
L-Glutamic Acid	20
Glycine	10
L-Histidine•HCl•H2O	20.2
Hydroxy-L-proline	20
L-Isoleucine	50
L-Leucine	50
L-Lysine•HCl	40
L-Methionine	15
L-Phenylalanine	15
L-Proline	20
L-Serine	30
L-Threonine	20
L-Tryptophan	5
L-Tyrosine•HCl	24
L-Valine	20

Vitamins

P-Amino Benzoic Acid	1
D-Biotin	0.2
Choline Chloride	3
Vitamin B-12	0.005
Folic Acid	1
i-Inositol	35
Niacinamide	1
D-Pantothenic Acid•Ca	0.25
Pyridoxine•HCl	1
Riboflavin	0.2
Thiamine•HCl	Absent

Other

Glucose	Absent
Glutathione	1
Phenol Red, Sodium	5

Total:

8.14g/L

References

1. Moore, G.E., Gerner, R.E. and Franklin, H.A., JAMA. 199, 519-524. (1967). 2. Moore, G.E. and Woods L.K., Tissue Culture Association Manual, 3, 503-508. (1976). 3. Moore, G.E. Gerner, R.E. and Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February, 41-63. (1967). 4. Moore, G.E. Gerner, R.E. and Kitamura,H.,. NY State Journal of Medicine. 68, 2054-2060. (1968)

USBio References

1. Zera K and Zastre J. 2017. Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes. PLoS One. 12(10):e0186707. doi: 10.1371/journal.pone.0186707. eCollection 2017.

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R9011-01 RPMI 1640 Medium Modified w/o L-Glutamine, Glucose, Thiamine•HCl (Powder)

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