Technical Data

R9016-L
Grade
Cell Culture Grade
Form
Magenta, clear, complete liquid
Shipping Temp
Blue Ice
Storage Temp
4°C
RPMI 1640 Medium Deficient w/o L-Glutamine, Methionine, Cystine (Liquid)

RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute.

pH
As Reported
Endotoxin
≤1EU/ml
Storage and Stability
Store at 4°C. Stable for 6 months after receipt.
Media Formulation
Components shown in mg/L
Inorganic Salts
Calcium Nitrate•4H2O100
Magnesium Sulfate48.8
Potassium Chloride400
Sodium Chloride6000
Sodium Phosphate Dibasic800.7
Amino Acids
L-Arginine200
L-Asparagine50
L-Aspartic Acid20
L-CystineAbsent
L-Glutamic Acid20
L-GlutamineAbsent
Glycine10
L-Histidine15
Hydroxy-L-proline20
L-Isoleucine50
L-Leucine50
L-Lysine•HCl40
L-MethionineAbsent
L-Phenylalanine15
L-Proline20
L-Serine30
L-Threonine20
L-Tryptophan5
L-Tyrosine•2Na•2H2O28.83
L-Valine20
Vitamins
p-Aminobenzoic Acid1
D-Biotin0.2
Choline Chloride3
Cyanocobalamin0.005
Folic Acid1
i-Inositol35
Nicotinamide1
D-Pantothenic Acid, Ca0.25
Pyridoxine•HCl1
Riboflavin0.2
Thiamine•HCl1
Other
D-Glucose2000
Glutathione,Reduced1
Phenol Red, Sodium5.3
Total:10g/L

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Moore, G.E., Gerner, R.E. and Franklin, H.A., JAMA. 199, 519-524. (1967). 2. Moore, G.E. and Woods L.K., Tissue Culture Association Manual, 3, 503-508. (1976). 3. Moore, G.E. Gerner, R.E. and Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February, 41-63. (1967). 4. Moore, G.E. Gerner, R.E. and Kitamura,H.,. NY State Journal of Medicine. 68, 2054-2060. (1968)
USBio References
No references available
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