Sabouraud Dextrose Agar w/Chloramphenicol (Powder)
Application
Selective for fungi isolation with inhibition of many bacteria.
Appearance
Beige, homogeneous free-flowing powder
Solubility: Visually the prepared medium is clear to trace hazy and light amber in color.
Microbiological Tests
Aspergillus niger ATCC 16404:Good Candida albicans ATCC 10231: Good Escherichia coli ATCC 25922: Inhibited Saccharomyces cerevisiae ATCC 18790: Good
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 65 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Casein Peptone | 5.0 |
| Dextrose | 40.0 |
| Meat Peptone | 5.0 |
| Agar | 15.0 |
| Chloramphenicol | 0.05 |
