Technical Data

S1013-60P
Grade
Affinity Purified
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity
Recognizes human Siglec 7.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Siglec 7 (Sialic Acid-binding Immunoglobulin-like Lectin, CDw328)

Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.
IHC Positive Control Tissue
Frozen tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Falco, M. et al. (1999) Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells. J. Exp. Med. 190: 793-801. 2. Nicoll, G. et al. (1999) Identification and characterization of a novel Siglec, Siglec-7, expressed by human natural killer cells and monocytes*. J. Biol. Chem. 274: 34089-34095.
USBio References
No references available
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