S1013-60P
Grade
Affinity PurifiedPurity
Purified by Protein G affinity chromatography from tissue culture supernatant.Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.Specificity
Recognizes human Siglec 7.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CSiglec 7 (Sialic Acid-binding Immunoglobulin-like Lectin, CDw328)
Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.
IHC Positive Control Tissue
Frozen tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.