STAB Agar (Powder)
Nutrient Broth, NaCl, Cysteine Hydrochloride, Thymine, Agar
Rich media for storage of bacterial strains.
Appearance
Light tan, homogeneous, free flowing powder
Solubility
Light yellow,, clear, complete after autoclaving
Directions per Liter
Dissolve 21.02 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. IDispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Components shown as g/liter |
| Agar | 6g |
| Cysteine Hydrochloride | 10mg |
| Nutrient Broth | 10g |
| Sodium chloride | 5g |
| Thymine | 10mg |
| Total: | 21.02g/L |
