Tryptic Soy Broth

Grade

Microbiological Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth

USP soybean-casein digest broth for sterility testing and general purpose use. Supports growth of fastidious and non-fastidious microorganisms such as Clostridia and non-sporulating anaerobes.

Suitable for Growth

Neisseria meningitides ATCC 13090 Streptococcus pneumoniae ATCC 6303 Streptococcus pyogenes ATCC 19615 Staphylococcus epidermidis ATCC 12228

Appearance

Light beige, homogenous, free flowing powder

Solubility

Lght amber, clear, complete

7.5 ± 0.2

Directions per Liter

Dissolve 30 grams per liter of ddH2O. Heat with stirring until dissolved. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.

Storage and Stability

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Media Formulation

Components shown as	g/liter
Dextrose	2.5
Potassium Phosphate Dibasic	2.5
Sodium Chloride	5.0
Enzymatic Digest of Soybean Meal	3.0
Pancreatic Digest of Casein	17.0

References

1. J.L.Smith,B.J.Dell,Capability of selective media to detect heat–injuredShigellaflexneri,J. Food Protect. 53, 141 (1990). 2. R.G. Garison,Studies of the respiratory activity of Histoplasma Capsulatum, J.ofinfect..Dis.108: 120-124 (1961). 3. N.B. McCulloug, Laboratory tests in the diagnosis of brucellosis. Amer.J.of pupli chealth 39:866-869 (1949)|4. Jean.F.MacFaddin, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins. (1985)

USBio References

No references available

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T8727-10 Tryptic Soy Broth (TSB) (Powder)

Grade

EU Commodity Code

Shipping Temp

Storage Temp

CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth

References

USBio References

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