T9058-01
Grade
Microbiological GradeForm
Off-white to beige, homogenous, free flowing powderEU Commodity Code
38210000Shipping Temp
RTStorage Temp
RT/4°CTryptose Phosphate Broth, Modified w/ Soytone, Yeast Extract (Powder)
Tryptose, Sodium Chloride, Sodium Phosphate, Dibasic, Glucose
Tryptose Phosphate Broth is a buffered dextrose broth for use as an adjuvant to tissue culture media and for the cultivation of fastidious bacteria. Ginsberg, et al., maintained tissue cultures of HeLa cells for at least 10 days in a mixture of 15-25% Tryptose Phosphate Broth, 67.5-77.5% Scheren maintenance solution and 7.5% chicken serum. The cells increased 3-5 fold in number during this period. Smaller quantities of ARD, AD and type 1 poliomyelitis virus could be detected and more ARD virus could be propagated in HeLa cells in the Tryptose Phosphate. Tryptose Phosphate Broth is recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms. Tryptose Phosphate Broth with added agar and sodium azide is recommended for the isolation of pathogenic streptococci from cheese and other dairy products (2,3). Tryptose Phosphate Broth with added agar is also recommended by the American Public Health Association for the examination of throat swabs and blood for Streptococcus pneumoniae and as a growth medium for pneumococci prior to the bile solubility test.
Appearance
Off-white to beige, homogenous, free flowing powder
Solubility
Yellow, clear, complete
Certificate of Origin
The raw animal product used in the manufacturing of Tryptose Phosphate Broth is derived from casein originating in the USA, New Zealand and/or Australia. During processing of the raw material, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes at 105°C. The product is then dried at a minimum of 140°C. BSE (Bovine Spongiform Encephalopathy) is not known to exist in the New Zealand and Australia.
Form
Off-white to beige, homogenous, free flowing powder
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 29.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Components shown as g/Liter |
Glucose | 2.0 |
Sodium Chloride | 5.0 |
Sodium Phosphate, Dibasic | 2.5 |
Soy Peptone | 5.0 |
Tryptone | 10.0 |
Yeast Extract | 5.0 |