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T9200 Two YT Broth (TY Broth) (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Tryptone, Yeast Extract, NaCl

Media for the propagation of M13 and filamentous bacteriophages in E. coli K12 strains, according to Miller (1).

BSE-Free
All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free
Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous
Proprietary blending technology ensures complete mixing of components
Appearance
Light tan to tan, homogeneous, free flowing powder
Solubility
Yellow to light orange, clear, complete
pH
7.0±0.2
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 31 grams n 900ml of ddH2O, stirring gently with heat with stirring until completely solubilized. Adjust pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as g/liter
Tryptone16
Yeast Extract B10
Sodium Chloride5
Total:31g/L
500g makes 16.1 liters
References
1. Miller, H., Meth. Enzymol. 152: 145 (1987). 2. Sun, D. & Setlow, P., J. Bacteriol. 173: 3831 (1991).
USBio References
No references available
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