For the cultivation of fungi, yeasts and other aciduric microorganisms such as Actinoplanes species, Streptomyces species, Streptoverticilium species and Nocardia species. Note:” Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.
Appearance
Tan, homogeneous, free flowing powder
Solubility
Llight yellow,, clear, complete
Directions per Liter
Dissolve 21 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Formulation per Liter | g/Liter |
Glucose | 10.0 |
Malt Extract | 3.0 |
Peptone | 5.0 |
Yeast Extract | 3.0 |