YEP Broth (Powder)
Standard media base for the maintenance and propagation of Schizosaccharomyces pombe and Saccharomyces cerevisiae. YEP Broth is YPD (YEPD) without dextrose and is used as a base for making a variation of YPD with an alternate carbon source.
Appearance
Tan to light yellow homogeneous, free flowing powder
Solubility
Yellow to amber, clear, complete
Directions per Liter
Dissolve 30 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Components shown as g/liter |
| Tryptone | 20g |
| Yeast Extract | 10g |
