YES Agar (Powder)
Media for the fission of Schizosaccharomyces pombe which allows for vegetative growth.
Appearance
Light tan, homogeneous and free flowing powder
Solubility
Light tan to light yellow, clear, complete after autoclaving
pH (after autoclaving)
As Reported
Directions per Liter
Dissolve 52.25 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Adenine | 0.05 |
| Agar | 17 |
| D-Glucose | 30 |
| Histidine | 0.05 |
| Leucine | 0.05 |
| Lysine | 0.05 |
| Uracil | 0.05 |
| Yeast Extract Y | 5 |
| Total: | 52.25g/L |
