YPDA, YEPD agar
Alternate formulation of the YPD series media, formulated with Peptone Y (casein digest, tryptone), useful for yeast propagation and plating of mutants and transformants.
Also includes the addition of an adenine hemisulfate supplement to a final 1X concentration of 120mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth.
Appearance
White to light tan, homogeneous, free flowing powder
Solubility
Amber, clear, complete after autovclaving
Directions per Liter
Dissolve 67.12g per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Yeast Extract | 10g |
| Peptone Y | 20g |
| Dextrose | 20g |
| Agar | 17g |
| Adenine Hemisulfate | 120mg |
| Total: | 67.12g/L |
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