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Y2077 YPD Broth Enhanced Formulation w/Peptone Y, Adenine (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
YEPD broth

Alternate formulation of the YPD series media, formulated with Peptone Y (casein digest, tryptone), useful for yeast propagation and plating of mutants and transformants.

Also includes the addition of an adenine hemisulfate supplement to a final 1X concentration of 120mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth.
Appearance
Light yellow to tan, homogeneous, free flowing powder
Solubility
Yellow-amber, clear, complete
pH
6.0-7.2
Directions per Liter
Dissolve 50 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Adenine Hemisulfate120mg
Dextrose20
Peptone Y20
Yeast Extract10
Total:50g/L
500g makes 10 liters
References
1. Ramage, G. et al., Rev Iberoam Micol (2008) 25: 37-40. 2. Ramage, G. et al., (2004) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 98:53-59. 3. Monteagudo, C. et al.,(2004) J Clin Pathol. 57:598–603. 4. Martins, M. et al., Mycopathologia (2009). 5. Zhang, H. and Seide, W. (2002) Nucleic Acids Research 30:1262-1267. 6. Harris, M.H. et al., (2000) Molecular and Cellular Biology 20:3590-3596. 7. Wasilenko, J.L. et al., (2010) Biotechnology Progress 26; 542-547. 8. Blake-Hodek, K. A. et al., (2010) Mol. Biol. Cell 21; 2013-2023.
USBio References
US Biological application references: 1. Ramage, G. et al., Rev Iberoam Micol (2008) 25: 37-40. 2. Ramage, G. et al., (2004) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 8:53-59. 3. Monteagudo, C. et al.,(2004) J Clin Pathol. 57:598–603. 4. Martins, M. et al., Mycopathologia (2009). 5. Zhang, H. and Seide, W. (2002) Nucleic Acids Research 30:1262-1267. 6. Harris, M.H. et al., (2000) Molecular and Cellular Biology 20:3590-3596. 7. Wasilenko, J.L. et al., (2010) Biotechnology Progress 26; 542-547. 8. Blake-Hodek, K. A. et al., (2010) Mol. Biol. Cell 21; 2013-2023.
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