012039
CAS Number
212118-77-9Grade
Highly PurifiedMDL Number
MFCD27977924Molecular Formula
C24H18As2O5S4Molecular Weight
664.5EU Commodity Code
38220090UN DOT Shipping
UN3465 PGIIIShipping Temp
Blue IceStorage Temp
-20°CFlAsH-EDT2
4',5'-Bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; FlAsH; Fluorescein Arsenical Helix Binder; Lumio Green
The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin.
Synonyms
4',5'-Bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; FlAsH; Fluorescein Arsenical Helix Binder; Lumio Green
Molecular Formula
C24H18As2O5S4
Appearance
White to pale orange solid
Melting Point
>173ºC (dec)
Solubility
Chloroform (Slightly, Heated), Methanol (Very Slightly, Heated)
Method for Determining Identity
Proton NMR (CDCl3) Spectroscopic and Mass Spectrometric Analysis
TLC Conditions
SiO2; Ethyl Acetate:Hexanes = 1:1; Visualized with UV and AMCS; SIngle Spot, Rf=0.50
Elemental Analysis
Calculated: %C: 43.38, %H: 2.73
Storage and Stability
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Light and temperature sensitive. Store under inert atmosphere.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gaietta, G., et al.: Science, 296, 503 (2002). 2. Hoffmann, C., et al.: Nature Methods, 2, 171 (2005). 3. Dyachok, O., et al.: Nature, 439, 349 (2006). 4. Gautier, A., et al.: Chem. Biol., 15, 128 (2008)USBio References
No references available