Technical Data

029631
Grade
Purified
Accession Number
NM_004359
Molecular Weight
29
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C
UbcH3, Active, Recombinant, Human, His-Tag (Ubiquitin-conjugating Enzyme E2 R1, Ubiquitin-conjugating Enzyme E2-32kD Complementing, Ubiquitin-conjugating Enzyme E2-CDC34, Ubiquitin-protein Ligase R1, CDC34, UBE2R1)

Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. UbcH3 has been shown to interact with a number of different E3 ligases, including SCFSkp2/Cks1, promoting the ubiquitination of p27 and SCFBTrCP1, promoting the ubiquitination of B-catenin and Ikba.

Full length recombinant corresponding to human UbcH3, fused to 6His-tag at N-terminal HA, expressed in E.coli.
Molecular Weight
~29kD
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Purity
≥84.2% (SDS-PAGE, Coomassie blue staining)
Concentration
~1mg/ml
Form
Supplied as a liquid in 50mM Tris/HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sadowski M. and Sarcevic B. Mechanisms of Mono- and Poly-Ubiquitination: ubiquitination specificity depends on compatibility between the E2 catalytic core and amino acid residues proximal to the lysine. Cell Division, 5: 19, 2010. 2. Semplici F. et al. CK2-Dependent Phosphorylation of the E2 Ubiquitin Conjugating Enzyme UBC3B Induces its Interaction with b-TrCP and Enhances b-catenin Degradation. Oncogene, 21: 3978-3987, 2002. 3. Wenzel D.M. et al. E2s: Structurally Economical and Functionally Replete. Biochem. J., 443: 31-42, 2011. 4. van Wijk S. J. L and Timmers H. T. M. The Family of Ubiquitin-Conjugating Enzymes (E2s):deciding between life and death of proteins. The FASEB Journal, 24: 981-993, 2010. 5. Xu S. et al. Substrate Recognition and Ubiquitination of SCFSkp2/Cks1 Ubiquitin-Protein Isopeptide Ligase. J Biol Chem. 282: 15462-15470, 2007. 6. Strack P. et al. SCFb-TRCP and Phosphorylation Dependent Ubiquitination of IκBa Catalyzed by Ubc3 and Ubc4. Oncogene, 19: 3529-3536, 2000.
USBio References
No references available
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