Progranulin, Recombinant, Human, FLAG-Tag (Proepithelin, PEPI, PC Cell-derived Growth Factor)
Proepithelin, PEPI, PC Cell-derived Growth Factor
Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer's Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.
Sequence
Signal peptide and human progranulin (aa1-593) are fused at the C-terminus to a FLAG®-tag.
Molecular Weight
~90kD (SDS-PAGE)
Biological Activity
Activates ERK phosphorylation in THP-1 and MCF10A cells and induces cell migration of THP-1 monocytic leukemia cells.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, HEK293 cells
Concentration
Not determined
Form
Supplied as a lyophilized powder in PBS. Reconstitute with sterile ddH2O.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
