Technical Data

048727
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
RT
Low Melting Point Agarose

Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. It is ideal for resolving DNA and RNA fragments and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C.

• Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Ideal for a variety of applications such as in-gel manipulation, tissue and cell culture, and viral plaque assays • Can also be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay)
Separation Range
100 to >30000 bp
Appearance
White, homogeneous, free flowing powder
Gel Strength (1%)
≥200g/cm
Electroendosmosis
0.15
Gel Temperature (1.5%)
24-30°C
Melting Point
65.5°C
Sulfate
≤0.10%
DNase and RNase
None Detected
Quality Control
Evaluated to satisfy specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase assay
Applications
Nucleic Acid Gel Electrophoresis, Protein Electrophoresis, Blotting
Storage and Stability
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
Form
White, homogeneous free flowing powder
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net