Technical Data

052069
Grade
Purified
Applications
E
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
Preservative Free
Yes
Notes
Preservative Free
Protein A

Binds the Fc portion of human IgG1, IgG2, and IgG4 as well as mouse IgG2a and IgG2b; also binds rabbit, porcine, guinea pig, and canine IgG.

Source
Staphylococcus aureus
Structure
Monomeric protein lacking cysteine residues; Mr 42kD
Applications
Suitable for use in ELISA. Though not tested, Western Blot, Purification and Immunoprecipitation have been reported in literature. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Staphylococcus aureus
Purity
Purified
Form
Supplied as a lyophilized powder from ddH2O. No preservative added. Reconstitute with 1ml sterile PBS.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sondgeroth K, Blitvich B, Blair C, Terwee J, Junge R, Sauther M, et al. Assessing flavivirus, lentivirus, and herpesvirus exposure in free-ranging ringtailed lemurs in southwestern Madagascar. J Wildl Dis. 2007;43:40-7. (ELISA)|2. Wycoff KL, Belle A, Deppe D, Schaefer L, Maclean JM, Haase S, et al. Recombinant anthrax toxin receptor-Fc fusion proteins produced in plants|protect rabbits against inhalational anthrax. Antimicrob Agents Chemother. 2011;55:132-9. (ELISA)|3. Duthie MS, Truman RW, Goto W, O'Donnell J, Hay MN, Spencer JS, et al. Insight toward early diagnosis of leprosy through analysis of the developing|antibody responses of Mycobacterium leprae-infected armadillos. Clin Vaccine Immunol. 2011;18:254-9. (ELISA)|4. Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Transient co-expression of post-transcriptional gene silencing suppressors for|increased in planta expression of a recombinant anthrax receptor fusion protein. Int J Mol Sci. 2011;12:4975-90. (ELISA)|5. Karuppanan K, Duhra-Gill S, Kailemia MJ, Phu ML, Lebrilla CB, Dandekar AM, et al. Expression, purification, and biophysical characterization of a|secreted anthrax decoy fusion protein in Nicotiana benthamiana. Int J Mol Sci. 2017;18:89. (ELISA)|6. Xiong Y, Li Q, Kailemia MJ, Lebrilla CB, Nandi S, McDonald KA. Glycoform modification of secreted recombinant glycoproteins through kifunensine|addition during transient vacuum agroinfiltration. Int J Mol Sci. 2018;19:890. (ELISA)|7. Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA. Transient recombinant protein production in glycoengineered Nicotiana|benthamiana cell suspension culture. Int J Mol Sci. 2018;19:1205. (ELISA)|8. Naskar P, Puri N. Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis. Biol Open. 2017;6:1257-69.|(WB)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net