TGFb1, Active, Recombinant, Human (Transforming Growth Factor Beta-1, TGF-beta-1, LAP, TGFB)
TGF beta is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. It plays an important role in bone remodeling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation, and differentiation in committed osteoblasts.
Recombinant protein corresponding to aa279-390 from human TGFb1 expressed in FreeStyle 293-F cells.
Biological Activity
Measured by TGFb1's ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells. The ED50 is <0.05ng/ml.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 0.2M Acetic acid with 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, FreeStyle 293-F cells
Concentration
Not determined
Form
Supplied as a lyophilized powder from 50mM glycine, 50mM sodium chloride buffer.Reconstitute with 0.2M acetic acid, 0.1% BSA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
