CRELD1, Recombinant, Human, aa30-362, His-Tag (Cysteine-rich with EGF-like Domain Protein 1, AVSD2, CIRRIN)
Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an ~50kD transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15aa (1). Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C-terminal ECD (1). CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro-apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4).
Source
Recombinant protein corresponding to aa30-362, Gln30-Glu362, from human CRELD1, fused to his-tag at C-terminal, NS0-derived.
Molecular Weight
~50-55kD
Endotoxin
<0.10EU/1ug (LAL method).
Biological Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.3-1.2ug/ml.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, NS0 cells
Concentration
~0.25mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder in PBS. Reconstitute in 500ul sterile PBS.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
