High Mobility Group AT Hook Protein 2 (HMGA2) Recombinant, Human
Recombinant protein corresponding to Met1-Asp109 with two N-Terminal Tags, His-Tag and GST-Tag from human HMGA2, expressed in E. coli. Uniprot/Accession: P52926.
Amino Acid Sequence
MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KPAQEETEET SSQESAEED
Accurate Molecular Weight
40kD as determined by SDS-PAGE reducing conditions
Predicted Molecular Weight
41.8kD
Predicted Isoelectric Point
7.7
Subcellular Location
Nucleus
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS, pH7.4 to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
