Matrix Metalloproteinase 9 (MMP9) Recombinant, Human
Recombinant protein corresponding to aa213-399 of human MMP9 with N-Terminal His-Tag expressed in E. coli. Uniprot/Acession: P14780.
Predicted Isoelectric Point
5.6
Predicted Molecular Mass
21.7kD
Accurate Molecular Mass
20kD as determined by SDS-PAGE reducing conditions.
Subcellular Location
Secreted, extracellular matrix
Amino Acid Sequence
GKGVVVPT RFGNADGAAC HFPFIFEGRSYSACTTDGRS DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP FTFLGKEYST CTSEGRGDGR LWCATTSNFD SDKKWGFCPD QGYSLFLVA
Applications
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
