Na-Cl Cotransporter (NCCT), Recombinant, Human
Source
Recombinant Human from E. coli
Fragment
Glu3~Cys146 (Accession No: P55017)
Sequence
MGHHHHHHSGSEF-ELPTTETPGD ATLCSGRFTI STLLSSDEPS PPAAYDSSHP SHLTHSSTFC MRTFGYNTID VVPTYEHYAN STQPGEPRKV RPTLADLHSF LKQEGRHLHA LAFDSRPSHE MTDGLVEGEA GTSSEKNPEE PVRFGWVKGV MIRC
Epitope Tag
N-terminal His-Tag
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
