Apo-SAA1, Recombinant, Human (Serum Amyloid A-1 Protein)
Serum amyloid A proteins (SAA) represents a family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). The level of Apo-SAA, normally 1-5ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apolipoprotein. The human SAA gene codes for a 122 amino acid nonglycosylated polypeptide, which contains an 18 amino acid N-terminal sequence. Recombinant human Apo-SAA1 is an 11.7kD protein containing 105 amino acid residues.
Recombinant protein corresponding to human Apo-Serum Amyloid A1 expressed in E. coli. Swiss/Uniprot: P02735
Amino Acid Sequence
MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY
Biological Activity
Determined by its ability to chemoattract human monocytes using a range of 10-100ng/ml.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder in 5mM Tris, pH7.6. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. For extended storage, the addition of 0.1% BSA is recommended. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
