USBio Logo

215378 Nerve Growth Factor (NGF, Beta NGF) CAS:

Specifications
References
Grade
Purified
Molecular Weight
2613
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of ~26,500Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF

Source
Nerve Growth Factor, from mouse
Molecular Weight
~26kD and the monomer ~13kD (SDS-PAGE)
Biological Activity
Tested by stimulation of 8 day old chick embryo dorsal root ganglia.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Mouse submaxillary glands
Purity
≥90% (SDS electrophoresis)
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder 0.1M acetic acid. Reconstitute with 100ul sterile ddH2O or PBS.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503. 2. Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54. 3. Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by γ-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24. 4. Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53.
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved